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KMID : 0613820170270010095
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Journal of Life Science
2017 Volume.27 No. 1 p.95 ~ p.99
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The Effect of Natural Compounds on the Longevity Extending in the Insect, Drosophila melanogaster
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Lee Jeong-Hoon
Kwon Ki-Sang
Lee Eun-Ryeong
Yoo Bo-Kyung
Ko Young-Hwa
Choi Ji-Young
Kwon O-Yu
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Abstract
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A previous study demonstrated that four kinds of natural compounds, Corydaline, (±)-Car-3-ene-2,5- dione, cinobufagin, and corilagin, enhanced the gene expression of ERAP1 and FOXO1 (DFA16) more than two-fold in a cell culture system. In this study, the experimental food was made finally 30 ml in which included 1% agar, 5% sucrose, and each natural compound 20 μl. A fruit fly of Drosophila melanogaster fed a natural product for 4 hr after 4 hr starvation. Both natural compounds of Cinobufagin and Corilagin induced 6-8 days more survival comparing than it controls group. The resulting fruit flies were estimated the gene expression of ERAP1 and FOXO1 by RT-PCR that also demonstrated meaningful results with the same lifespan results. Cinobufagin from BufonisVenennumis has C26H34O6 molecular formula and 442 kDa molecular weight. Corilagin from Euphorbiapekinensisis has C27H22O18 molecular formula and 634 kDa molecular weight. The two types of natural products screened in this study will be used in the early diagnosis and treatment of insect industry in the near future. In addition, the natural products will be used in longevity experiments in a mouse model. The results may give one of the clues for studying new drug development candidates of the longevity.
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KEYWORD
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Cinobufagin, Corilagin, D.melanogaster, ERAP1, FOXO1
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